Chromatrap allows you to update your ChIP and library preparation with the Chromatrap kit, by letting you sequentially perform ChIP and library preparation for next-generation sequencing. So, introducing the UniqSeq, the Chromatrap® UniqSeq kit combines its revolutionary solid-state technology and next-generation buffer chemistry to deliver a faster and more efficient workflow from start to finish.

With the UniqSeq kit, you will obtain high-quality libraries low cell numbers from a wide range of samples. Be ready-to-sequence in just under 8 hours. The UniqSeq kit is part of the faster, easier, and more sensitive kits for ChIP.

There are many features associated with the UniSeq kit such as its:

• Cost-effective
• It has a 1-tube reaction
• It can be validated in ChIP
• It has an integrated solid-state system
• It has a high-fidelity PCR mix
• Indexing primers for Illumina included

Not only it is cost-effective with all the reagents you need from start to finish – 24 ChIP’s, 24 library preparation, 12 indices. But the 1-tube reaction has end-repair, an A-tail and ligate in a single step, which means there is no clean-up required between responses.

Furthermore, since it is validated in ChIP, it can prepare libraries from any ChIP sample with ease and reassurance. It is a leading bead-free system for performing highly sensitive, capable ChIP with ease with the flexibility of size selection with bead-free workflow.

The Uniq ChIP kit also allows you to minimise GC bias and obtain high library yields with high fidelity of the original sample. You can create high quality, complex libraries with low duplication rates.

Other Uniq ChIP kits which are part of the library preparation kits and supplied by Chromatrap include:

• The UniqSeq Sonication Pro A
• The UniqSeq Sonication Pro G
• The UniqSeq Sonication Pro A
• The UniqSeq Sonication Pro G

The UniqSeq components and reagents are fascinating. Within the UniqSeq Sonication Pro A product you will find ChIP reagents such as:

• Chromatrap® spin columns (24)
• 5 ml Collection Tube (50)
• Chromatrap® DNA purification columns (50)
• Column Conditioning Buffer, Wash Buffers (1, 2, 3)
• Glycine (1.3M)
• Lysis Buffer
• ChIP-seq Elution Buffer
• NaCl (5M)
• NaHCO3 (1M)
• Hypotonic Buffer
• H3K4me3 antibody
• Immunoglobulin G
• Protease Inhibitor Cocktail
• Forward & Reverse Primers

And many more including the Proteinase K and its stop solution, and DNA Binding Buffer etc.

The library preparation kit reagents of The UniqSeq Sonication Pro A Include many such as the:

• End Prep and Tail Enzyme
• End Prep and Tail Buffer
• Ligation Mix
• Adapter Ligase
• Adapter for Illumina®
• Loop Restriction Enzyme
• Library Enrich and Index Mix
• I5 primer for Illumina®
• Index primers 1-12 for Illumina®

There are many advantages of a bead-free kit including a user-friendly experience due to a vast reduction of manual handling steps involved, significantly reduced sample loss between levels and no background, with the aid of the bead-free inert filter technology. The ChIP-seq kits can also complete a ChIP assay in under 5 hours.

Get in touch with the team at Chromatrap today if you are interested in the UniqSeq kit or any library preparation kit and ChIP seq buffers. You can call them today on +44 (0)1978 661144 and speak with a representative to assist you further, or you can email [email protected]. One other way to get in touch or to find out more information about the Chromatrap products, you can go online and visit their website at https://www.chromatrap.com/chip-kits/.

Source: https://thenewsfront.com/chromatrap-allows-you-to-update-your-chip-and-library-preparation-with-the-chromatrap-kit/